Mushroom Substrate Kit XL | Sterile Substrate for mushroom cultivation

You can think of the sterilized mushroom substrate as soil for a plant. We need to inoculate this sterilized substrate with our liquid cultures derived from spores.

The mushroom substrate kit contains

• The container contains a 2100 cc/ml sterile substrate mix.
• Liquid Culture 1 x 25 ml
• Injection port
• Fruiting Bag with Filter
• Alcohol swap
• Clip

Substrate Contains:

The substrate is sterilized and has a moisture content of 60–65%. The mixture consists of whole oat, vermiculite, perlite, and Gypsum.

Recommeded products to use

• Liquid Culture
• Spore syringe
• Spore Vial
• Face mask
• Gloves
• Lighter
• Desinfectant handgel

Storage

Always store your substrate kit in a clean refrigerator! We recommend using the substrate kit as soon as possible. You can refrigerate the kit for up to 6 weeks.

The instructions for using a Mushroom Substrate Kit are available here.

Phase 1: Prepare the Liquid Culture

Wash your hands and clean the working area!

1. Shake the spore vial or syringe very well to make sure all spores are floating.
2. Disinfect the injection site of the liquid culture and the spore vial or syringe with the alcohol swap.
3. Inject 2 ml of spore solution into the liquid culture.

Shake the inoculated liquid culture vigorously once a day every day for 10 days. Store the inoculated liquid culture in a place between 23 and 27 degrees Celsius. The liquid culture is ready when you see a cloud floating in the suspension. This is mycelium.

Your liquid culture, known as the Master Culture, is ready. With proper use and storage, it can produce a large amount of mycelium from a small amount of spores.

Important!

• If the liquid is murky, it means there is contamination and the culture is unusable.
• If you don’t use the liquid culture for 10 days, store it in the refrigerator.

Phase 2: Inoculation

Step 1

• Wash your hands and clean your working area.
• The injection site of the sterilized substrate and liquid culture should be clean.

Step 2

• Retract 10 ml (1200 CC kit) or 25 ml (2100 CC) from the liquid culture.

• Inject spore suspension through the injection site of the kit.

Step 3

• After inoculation, turn and shake the box 360 degrees in all directions.
• Store the kit in a dark place with a temperature between 23°C and 27°C.

Result:

After inoculation, you’ll see mycelium form. A white fungus will gradually fill the sterilized substrate. This can take up to 2-3 weeks, depending on the temperature.

Phase 3: Fruiting and Spore Collection

Please keep in mind that fruiting mushrooms can be illegal in your country. Always check your local laws before entering this stage.

Once you’ve fully colonized the substrate, it’s time to start harvesting fruits to gather mushroom spores or tissue for further research or cultivation.