Sterile Magic Mushroom substrate for p. Cubensis Large


Mushroom Substrate Large Kit includes 1200 ml of sterile mushroom substrate. This substrate package is tailored to the requirements of the Cubensis mushroom. You add the spores and develop any strain you want. For improved spore development, we recommend using Liquid Culture, which is included with the package. Do you require spores? Adding Spores will give you an additional 15% off. Simply add a spores Syringe / Vial to your purchase to receive the discount. You are in command. Create your own Magic!

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Sterile Magic Mushroom substrate for p. Cubensis Large

Magic Mushroom Substrate Kit Large For Mushroom Cultivation | Sterile Substrate for Mushroom Culture. The mushroom-sterilised substrate can be compared to soil for plants. This sterile substrate must be injected with our spore-derived liquid cultures.

Mushroom Substrate Kit contains

  • Container with 1200 cc / ml sterile substrate mix
  • Liquid Culture 10 ml
  • Injection port
  • Fruiting Bag with Filter
  • Alcohol swap
  • Clip

Substrate Contains:

Sterilised substrate with a moisture content: 60-65%. A mixture of whole oat, vermiculite, perlite and Gypsum.

Recommeded products to use

  • Spore syringe
  • Spore Vial
  • Face mask
  • Gloves
  • Lighter
  • Desinfectant handgel


Always store your substrate kit in a clean refrigerator ! We recommend to use the substrate kit as soon as possible. The kit can be kept under refrigerated conditions for up to 6 weeks.

How to use a Mushroom Substrate Kit : Instructions

Phase 1 – Prepare the Liquid Culture

Wash your hands and clean the working area! .

  1. Shake the spore vial or syringe very good to make sure all spores are floating.
  2. Disinfect the injection site of the liquid culture and the spore vial or syringe with the alcohol swap.
  3. Inject 2 ml of spore solution in the Liquid culture

For 10 days, firmly shake the infected liquid culture once a day. Keep the inoculated liquid culture at a temperature of 23-27 degrees Celsius. When you notice a cloud floating in the suspension, the liquid culture is ready.

Mycelium is what it’s called. Your liquid culture is complete; it is known as the Master Culture, and if used and preserved properly, it may produce a large amount of mycelium from a small amount of spores.


  • If the liquid is murky, it means there is a contamination and the culture is unusable.
  • If you don’t use the liquid culture for 10 days, store it in the refrigerator

Phase 2 – Inoculation

Step 1

  • Wash your hands and clean your working area
  • Clean injection site of the sterilized substrate and liquid culture

Step 2

  • Retract 10ml (1200 CC kit) or 10 ml (2100 CC) from the liquid culture
  • Inject spore suspension, through the injection site of the kit

Step 3

  • After inoculation turn and shake the box 360 degrees in all directions
  • store the kit in a dark place with temperature between 23 °-27°C


Mycelium will form after inoculation. The sterilized substrate will eventually fill with a white fungus. Depending on the temperature, this could take up to 2-3 weeks.

Phase 3 – Fruiting and Spore collection

Please bear in mind that in your nation, fruiting mushrooms may be illegal. Before proceeding, always verify your local laws. When the substrate has been completely colonized, it is time to start growing the fruits so that you can harvest mushroom spores or mushroom tissue to continue your research or cultivation.


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